The Rotor-Gene Q is engineered to reduce the need for maintenance and to maximize ease of use. A specially tuned high-intensity optical HRM channel.The HRM option for the Rotor-Gene Q includes: The rotary design of the Rotor-Gene Q and its outstanding thermal and optical performance are highly suited to HRM. Previously unknown and even complex sequence variations can be readily detected and characterized in a robust and straightforward way. PCR products can be discriminated according to sequence, length, GC content, or strand complementarity, down to single base-pair changes. It is similar to classical melting curve analysis, but provides far more information for a wider range of applications. HRM characterizes double-stranded PCR products based on their dissociation (melting) behavior. High-resolution melting analysis (HRM) is a closed-tube, post-PCR analysis that has raised enormous scientific interest. In addition, the software allows you to create new excitation/detection wavelength combinations, which means that the Rotor-Gene Q is compatible with dyes you may use in the future. With up to 6 channels spanning UV to infrared wavelengths, the cycler delivers the widest optical range currently available (see table). Whether your assay is based on intercalating dyes such as SYBR ® Green, probes such as hydrolysis (TaqMan®), hybridization (FRET), Scorpion probes, or other multiplex chemistries, the Rotor-Gene Q meets your requirements. Unrivaled optical range enables multiple applications
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